| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes
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| Autores: |
Samain, Jean-francois
Degremont, Lionel
Soletchnik, Patrick
Haure, Joel
Bedier, Edouard
Ropert, Michel
Moal, Jeanne
Huvet, Arnaud
Bacca, Helene
Van Wormhoudt, A
Delaporte, Maryse
Costil, K
Pouvreau, Stephane
Lambert, Christian
Boulo, Viviane
Soudant, Philippe
Nicolas, Jean-louis
Leroux, Frederique
Renault, Tristan
Gagnaire, Beatrice
Geret, Florence
Boutet, Isabelle
Burgeot, Thierry
Boudry, Pierre
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| Data: |
2007-08
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| Ano: |
2007
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| Palavras-chave: |
ROS
Temperature
Stress
Hemocytes
Reproduction
Genetic selection
Resistant oyster
Crassostrea gigas
Pacific Oyster
Summer mortality
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| Resumo: |
Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials confirming the high heritability of survival of juveniles < 1 year old. Interestingly, no correlation was observed between growth and survival. Comparison between the two selected groups showed that S oysters invested more energy in reproduction and stayed a longer time without spawning than R oysters which had high synchronous spawning. This was mainly shown with high rather than low dietary rations (respectively 12% and 4% DW algae/DW oyster) in a controlled experiment. Moreover, early partial spawning was detected in S oysters and not R ones in the high dietary ration. S showed a higher respiration rate and an earlier decrease in absorption efficiency than R during gametogenesis, but they were not significantly different in glycogen or ATP utilisation. Two months before a mortality episode, hemocytes from S oysters had a higher adhesive capacity than R hemocytes and significantly higher reactive oxygen species production capacity. One month before mortality, S oysters had the highest hyalinocyte concentration and their expression of genes coding for glucose metabolism enzymes (Hexokinase, GS, PGM, PEPCK) was significantly lower in the labial palps. After a thermal increase from 13 °C to 19 °C, during 8 days in normoxia, S oysters showed a large HSP70 increase under hypoxia contrary to R oysters, suggesting their high susceptibility to stress. Their catalase activity was lower than in R oysters and showed no further change to subsequent hypoxia and pesticide stresses, in contrast to R oysters. These observations suggest possible links between higher reproductive effort in S oysters, their specific stress response to temperature and hypoxia, ROS production, partial spawning, hyalinocyte increase and the infection process. To compare R and S oysters in a more integrated way, a suppression subtractive hybridisation (SSH) library and a micro-array strategy are being undertaken.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2007/publication-2809.pdf
DOI:10.1016/j.aquaculture.2007.04.044
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/2809/
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| Formato: |
application/pdf
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| Fonte: |
Aquaculture (0044-8486) (Elsevier), 2007-08 , Vol. 268 , N. 1-4 , P. 227-243
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| Direitos: |
Elsevier B.V. All rights reserved
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